The NextSeq system brings the power of a high-throughput sequencing system to your benchtop. With tunable output and high data quality, it provides the flexible power you need for whole-genome, transcriptome, and targeted resequencing. Perform many combinations of high- and mid-throughput sequencing applications to advance your studies. The NextSeq can achieve 16GB – 120GB outputs with 130 – 400 million reads per run. Available to run single or paired end sequencing protocols of either 75 or 150 base read lengths. Our core center is home to two of these Illumina NextSeq systems!
Applications:
Equipment Rates:
| MiSeq Service | Internal Academic Rate | Single Reads/Read Pairs | Yield |
|---|---|---|---|
| Nano 300-Cycle | Inquire | Up to 1 Million | Up to 300 Mb |
| Nano 500-Cycle | Inquire | Up to 1 Million | Up to 500 Mb |
| Micro 300-Cycle | Inquire | Up to 4 Million | Up to 1.2 Gb |
| V2 50-Cycle | Inquire | Up to 15 Million | Up to 850 Mb |
| V2 300-Cycle | Inquire | Up to 15 Million | Up to 5.1 Gb |
| V2 500-Cycle | Inquire | Up to 15 Million | Up to 8.5 Gb |
| V3 150-Cycle | Inquire | Up to 25 Million | Up to 3.8 Gb |
| V3 600-Cycle | Inquire | Up to 25 Million | Up to 15 Gb |
| NextSeq 550 Service | Internal Academic Rate | Single Reads/Read Pairs | Yield |
| 75-Cycle High Output | Inquire | Up to 400 Million | Up to 30 Gb |
| 150-Cycle Mid Output | Inquire | Up to 130 Million | Up to 20 Gb |
| 150-Cycle High Output | Inquire | Up to 400 Million | Up to 60 Gb |
| 300-Cycle Mid Output | Inquire | Up to 130 Million | Up to 40 Gb |
| 300-Cycle High Output | Inquire | Up to 400 Million | Up to 120 Gb |
| NextSeq 2000 Service | Internal Academic Rate | Single Reads/Read Pairs | Yield |
| 50-Cycle P3 | Inquire | Up to 1.2 Billion | Up to 60 Gb |
| 100-Cycle P1 | Up to 100 Million | Up to 10 Gb | |
| 100-Cycle P2 | Up to 400 Million | Up to 40 Gb | |
| 100-Cycle P3 | Up to 1.2 Billion | Up to 120 Gb | |
| 200-Cycle P2 | Up to 400 Million | Up to 80 Gb | |
| 200-Cycle P3 | Up to 1.2 Billion | Up to 240 Gb | |
| 300-Cycle P1 | Up to 100 Million | Up to 60 Gb | |
| 300-Cycle P2 | Up to 400 Million | Up to 120 Gb | |
| 300-Cycle P3 | Up to 1.2 Billion | Up to 360 Gb | |
| 600-Cycle P1 | Up to 100 Million | Up to 60 Gb | |
| 600-Cycle P2 | Up to 300 Million | Up to 180 Gb | |
| NovaSeq 6000 Service | Internal Academic Rate | Single Reads/Read Pairs | Yield |
| 35-Cycle S4 | Inquire | Up to 10 Billion | Up to 350 Gb |
| 100-Cycle SP | Up to 800 Million | Up to 80 Gb | |
| 100-Cycle S1 | Up to 1.6 Billion | Up to 167 Gb | |
| 100-Cycle S2 | Up to 4.1 Billion | Up to 417 Gb | |
| 200-Cycle SP | Up to 800 Million | Up to 167 Gb | |
| 200-Cycle S1 | Up to 1.6 Billion | Up to 333 Gb | |
| 200-Cycle S2 | Up to 4.1 Billion | Up to 833 Gb | |
| 200-Cycle S4 | Up to 10 Billion | Up to 2000 Gb | |
| 300-Cycle SP | Up to 800 Million | Up to 250 Gb | |
| 300-Cycle S1 | Up to 1.6 Billion | Up to 500 Gb | |
| 300-Cycle S2 | Up to 4.1 Billion | Up to 1250 Gb | |
| 300-Cycle S4 | Up to 10 Billion | Up to 3000 Gb | |
| 500- Cycle SP | Up to 800 Million | Up to 180 Gb | |
| Add-On Service | Internal Academic Rate | ||
| Library Quality Analysis (Qubit, Fragment Analysis, ddPCR) | |||
| Library Pooling | |||
| NovaSeq Xp 2-Lane Kit for lane-splitting | |||
| NovaSeq Xp 4-Lane Kit for lane-splitting |